This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0040128
PrimePCR™ PreAmp for SYBR® Green Assay: GOLGA6B, Human
PrimePCR™ Template for SYBR® Green Assay: GOLGA6B, Human
This gene is found in a large low copy repeat sequence or duplicon that is found in multiple copies which are greater than 90% similar on chromosome 15. Duplicons are associated with deletions inversions and other chromosomal rearrangements that underlie genomic disease. This gene is a member of the golgin gene family whose protein products localize to the Golgi apparatus. The majority of the related gene copies are thought to be transcribed pseudogenes. It is not known whether this gene is a pseudogene or if it encodes a golgin protein. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.