PrimePCR™ SYBR® Green Assay: FRG2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040101

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FRG2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FRG2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037378
Assay Design:   exonic
Chromosome Location:   4:190946626-190946742question
Amplicon Length:   87
Splice Variants Targeted:   ENST00000234142 ENST00000432985 ENST00000381486 ENST00000569512 ENST00000563327 ENST00000443774 ENST00000368078 ENST00000467691 ENST00000368079 ENST00000504750 ENST00000378763 ENST00000464571 ENST00000308062 ENST00000403385

Gene Information

Description Not Available

Gene Symbol:   FRG2
Gene Name:   FSHD region gene 2
Aliases:   FRG2A
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000205097
Entrez:   448831
Chromosome Mapping:   4q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.470000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download