PrimePCR™ SYBR® Green Assay: FOXD4L6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040093

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037370
Assay Design:   exonic
Chromosome Location:   9:69201864-69201982question
Amplicon Length:   89
Splice Variants Targeted:   ENST00000292478 ENST00000327451 ENST00000399813 ENST00000349780 ENST00000360190 ENST00000416449 ENST00000360822 ENST00000359309 ENST00000377590 ENST00000251091 ENST00000392067 ENST00000357634 ENST00000377473 ENST00000377424 ENST00000377420 ENST00000377413 ENST00000342833 ENST00000435422 ENST00000577592

Gene Information

Description Not Available

Gene Symbol:   FOXD4L6
Gene Name:   forkhead box D4-like 6
Aliases:   MGC119257
RefSeq:   NC_000009.11 NT_078070.3
Ensembl:   ENSG00000204793
Entrez:   653404
UniGene:   Hs.652377
Chromosome Mapping:   9q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.820000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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