PrimePCR™ SYBR® Green Assay: MUC21, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040066

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MUC21, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MUC21, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037343
Assay Design:   exonic
Chromosome Location:   6:30955914-30956004question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000584437 ENST00000457728 ENST00000547599 ENST00000430305 ENST00000547796 ENST00000420532 ENST00000549059 ENST00000354618 ENST00000469141 ENST00000440457 ENST00000551445 ENST00000486149 ENST00000376296 ENST00000451480 ENST00000548519 ENST00000383535 ENST00000551904 ENST00000358136 ENST00000356315 ENST00000011700 ENST00000220244 ENST00000394685 ENST00000356249 ENST00000301011 ENST00000452588 ENST00000567085

Gene Information

Description Not Available

Gene Symbol:   MUC21
Gene Name:   mucin 21, cell surface associated
Aliases:   C6orf205, KMQK697, MGC125609, MUC-21, bCX31G15.2
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000204544
Entrez:   394263
Chromosome Mapping:   6p21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.030000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download