PrimePCR™ SYBR® Green Assay: PRAMEF9, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040056

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037333
Assay Design:   exonic
Chromosome Location:   1:13427344-13427481question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000267868 ENST00000398117 ENST00000417046 ENST00000064778 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000355096 ENST00000376192 ENST00000376189 ENST00000415464 ENST00000376182 ENST00000414205 ENST00000423177 ENST00000376168 ENST00000376152 ENST00000327795 ENST00000332192 ENST00000330087 ENST00000602960 ENST00000316412 ENST00000372833 ENST00000401694

Gene Information

Description Not Available

Gene Symbol:   PRAMEF9
Gene Name:   PRAME family member 9
Aliases:   PRAMEF15
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000204501
Entrez:   343070
UniGene:   Not Available
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.993900
y-intercept 34.830000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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