PrimePCR™ SYBR® Green Assay: PRAMEF18, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040053

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRAMEF18, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRAMEF18, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037330
Assay Design:   exonic
Chromosome Location:   1:13476372-13476504question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000402868 ENST00000330479 ENST00000263686 ENST00000367786 ENST00000458599 ENST00000367788 ENST00000367793 ENST00000367794 ENST00000367792 ENST00000367791 ENST00000376187 ENST00000353410 ENST00000376173 ENST00000376126 ENST00000376101 ENST00000540591 ENST00000372548 ENST00000372544

Gene Information

Description Not Available

Gene Symbol:   PRAMEF18
Gene Name:   PRAME family member 18
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000204491
Entrez:   391003
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.810000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download