PrimePCR™ SYBR® Green Assay: STK19, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040027

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: STK19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: STK19, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037304
Assay Design:   exonic
Chromosome Location:   6:31940079-31940171question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000426802 ENST00000452688 ENST00000549310 ENST00000425138 ENST00000433397 ENST00000551718 ENST00000383328 ENST00000383327 ENST00000547578 ENST00000460018 ENST00000375331 ENST00000375333 ENST00000424104 ENST00000438256 ENST00000550656 ENST00000444147 ENST00000431383 ENST00000549916

Gene Information

This gene encodes a serine/threonine kinase which localizes predominantly to the nucleus. Its specific function is unknown; it is possible that phosphorylation of this protein is involved in transcriptional regulation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6 and expresses two transcript variants. [provided by RefSeq Jul 2008]

Gene Symbol:   STK19
Gene Name:   serine/threonine kinase 19
Aliases:   D6S60, D6S60E, G11, HLA-RP1, MGC117388, RP1
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NG_011638.1 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204344
Entrez:   8859
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.270000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download