PrimePCR™ SYBR® Green Assay: PRRT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040025

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PRRT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PRRT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037302
Assay Design:   exonic
Chromosome Location:   6:32116330-32116444question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000458719 ENST00000441129 ENST00000551213 ENST00000444466 ENST00000418753 ENST00000552325 ENST00000370225 ENST00000451329 ENST00000445122 ENST00000552684 ENST00000419655 ENST00000423671 ENST00000548904 ENST00000454112 ENST00000383309 ENST00000549696 ENST00000456814 ENST00000425739 ENST00000551958 ENST00000301149 ENST00000414687 ENST00000452030 ENST00000547412 ENST00000211413 ENST00000375150 ENST00000375152

Gene Information

Description Not Available

Gene Symbol:   PRRT1
Gene Name:   proline-rich transmembrane protein 1
Aliases:   C6orf31, IFITMD7, NG5
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204314
Entrez:   80863
Chromosome Mapping:   6p21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.240000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download