PrimePCR™ SYBR® Green Assay: RIMBP3B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039984

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037261
Assay Design:   exonic
Chromosome Location:   22:21742629-21742742question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000411734 ENST00000355186 ENST00000426804 ENST00000357029 ENST00000434111 ENST00000331505 ENST00000433039 ENST00000537591 ENST00000438527 ENST00000550722 ENST00000377560 ENST00000430131

Gene Information

Description Not Available

Gene Symbol:   RIMBP3B
Gene Name:   RIMS binding protein 3B
Aliases:   RIM-BP3.2, RIM-BP3.B, RIMBP3.2
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000196934
Entrez:   440804
UniGene:   Hs.568257
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.940000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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