PrimePCR™ SYBR® Green Assay: FFAR3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039937

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037214
Assay Design:   exonic
Chromosome Location:   19:35850076-35850170question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000361719 ENST00000392299 ENST00000449475 ENST00000327809 ENST00000594310 ENST00000454971 ENST00000597214 ENST00000354534 ENST00000355133 ENST00000545061

Gene Information

Description Not Available

Gene Symbol:   FFAR3
Gene Name:   free fatty acid receptor 3
Aliases:   FFA3R, GPR41
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000185897
Entrez:   2865
UniGene:   Hs.248055
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.940000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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