PrimePCR™ SYBR® Green Assay: AQP12B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039933

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AQP12B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AQP12B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037210
Assay Design:   exonic
Chromosome Location:   2:241622185-241622274question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000547334 ENST00000552264 ENST00000549001 ENST00000549105 ENST00000549322 ENST00000552021 ENST00000550392 ENST00000447614 ENST00000307641 ENST00000393885 ENST00000393884 ENST00000339474 ENST00000400877 ENST00000334962 ENST00000584306 ENST00000319651 ENST00000407834 ENST00000337801 ENST00000429564 ENST00000343632 ENST00000381756 ENST00000325180 ENST00000406772 ENST00000337437 ENST00000569942 ENST00000562265

Gene Information

Description Not Available

Gene Symbol:   AQP12B
Gene Name:   aquaporin 12B
Aliases:   INSSA3, MGC149473
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000185176
Entrez:   653437
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download