This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039918
PrimePCR™ PreAmp for SYBR® Green Assay: TCEB3C, Human
PrimePCR™ Template for SYBR® Green Assay: TCEB3C, Human
The SIII (or elongin) transcription elongation factor complex stimulates the rate of transcription elongation by RNA polymerase II by suppressing the transient pausing of the polymerase at many sites along the DNA template. This complex is a heterotrimer composed of the transcriptionally active subunit A A2 or A3 (or elongin A A2 or A3) and two regulatory subunits B and C (or elongin B and C). This gene encodes subunit A3. A3 and A are ubiquitously expressed whereas A2 is specifically expressed in the testis. [provided by RefSeq Mar 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.