PrimePCR™ SYBR® Green Assay: HIST2H3D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039913

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: HIST2H3D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: HIST2H3D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037190
Assay Design:   exonic
Chromosome Location:   1:149784866-149784956question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000340398 ENST00000540144 ENST00000356476 ENST00000377831 ENST00000360408 ENST00000291495 ENST00000586018 ENST00000331491 ENST00000369158 ENST00000403683 ENST00000297954 ENST00000432730 ENST00000448251 ENST00000453718 ENST00000395477 ENST00000395475 ENST00000349097 ENST00000427277 ENST00000356055 ENST00000366696 ENST00000254810 ENST00000592643 ENST00000591890 ENST00000587560 ENST00000589599 ENST00000586607 ENST00000586270 ENST00000578851 ENST00000585113 ENST00000580481

Gene Information

Description Not Available

Gene Symbol:   HIST2H3D
Gene Name:   histone cluster 2, H3d
Aliases:   HIST2H3A, HIST2H3C
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000183598
Entrez:   653604
Chromosome Mapping:   1q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.160000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download