PrimePCR™ SYBR® Green Assay: GGTLC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039906

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GGTLC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GGTLC3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037183
Assay Design:   exonic
Chromosome Location:   22:20366362-20366827question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000248923 ENST00000400382 ENST00000400380 ENST00000401885 ENST00000404532 ENST00000403838 ENST00000404223 ENST00000400383 ENST00000406383 ENST00000404912 ENST00000424787 ENST00000308128 ENST00000359747 ENST00000360001 ENST00000263741 ENST00000405188 ENST00000401924 ENST00000424627 ENST00000286890 ENST00000278765 ENST00000335694 ENST00000511647 ENST00000452328 ENST00000512960 ENST00000413543 ENST00000226319 ENST00000378944 ENST00000314737 ENST00000592958 ENST00000587447 ENST00000588248 ENST

Gene Information

Description Not Available

Gene Symbol:   GGTLC3
Gene Name:   gamma-glutamyltransferase light chain 3
Aliases:   GGT
RefSeq:   NC_000022.10 NG_011826.1 NT_011519.10
Ensembl:   ENSG00000183038
Entrez:   728226
Chromosome Mapping:   22q11.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.290000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download