PrimePCR™ SYBR® Green Assay: FAM27A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039902

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FAM27A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FAM27A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037179
Assay Design:   exonic
Chromosome Location:   9:45728132-13388459question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000511828 ENST00000335753 ENST00000369755 ENST00000302909 ENST00000344721 ENST00000358102 ENST00000512865 ENST00000342437 ENST00000511528 ENST00000355292 ENST00000219837 ENST00000561100 ENST00000377542 ENST00000377531 ENST00000417276 ENST00000377484 ENST00000218176 ENST00000467517 ENST00000546625 ENST00000301873 ENST00000593940

Gene Information

Description Not Available

Gene Symbol:   FAM27A
Gene Name:   family with sequence similarity 27, member A
Aliases:   FAM27A1, MGC11241, bA7G23.5
RefSeq:   NC_000009.11 NT_078053.4
Ensembl:   ENSG00000182368
Entrez:   548321
Chromosome Mapping:   9q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download