PrimePCR™ SYBR® Green Assay: DEFB103B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039890

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DEFB103B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DEFB103B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037167
Assay Design:   exonic
Chromosome Location:   8:7286477-7286603question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000314357 ENST00000227135 ENST00000532692 ENST00000354685 ENST00000527990 ENST00000254579 ENST00000318124 ENST00000396716

Gene Information

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin beta 103B which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq Jul 2008]

Gene Symbol:   DEFB103B
Gene Name:   defensin, beta 103B
Aliases:   BD-3, DEFB103, DEFB103A, DEFB3, HBD3, HBP3
RefSeq:   NC_000008.10 NT_023736.17
Ensembl:   ENSG00000177243
Entrez:   55894
Chromosome Mapping:   8p23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.650000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download