PrimePCR™ SYBR® Green Assay: RP11-330H6.5, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039878

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RP11-330H6.5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RP11-330H6.5, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037155
Assay Design:   exonic
Chromosome Location:   3:52255124-52255229question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000360658 ENST00000494383 ENST00000446197 ENST00000359827 ENST00000321063 ENST00000416464 ENST00000417257 ENST00000402883 ENST00000424445 ENST00000449552 ENST00000371322 ENST00000336592 ENST00000404115 ENST00000360535 ENST00000593074 ENST00000250572 ENST00000381407 ENST00000312053 ENST00000450315 ENST00000381404 ENST00000229022 ENST00000550325 ENST00000549336 ENST00000395324 ENST00000598777

Gene Information

Description Not Available

Gene Symbol:   RP11-330H6.5
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000173366
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.170000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download