PrimePCR™ SYBR® Green Assay: TSPY2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039853

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037130
Assay Design:   exonic
Chromosome Location:   Y:6116914-89354939question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000320701 ENST00000383042 ENST00000426950 ENST00000383008 ENST00000287721 ENST00000383000 ENST00000383005 ENST00000330628 ENST00000457222 ENST00000424594 ENST00000440483 ENST00000427871 ENST00000451548 ENST00000423647 ENST00000440215 ENST00000446779 ENST00000428845 ENST00000444056 ENST00000429039 ENST00000292782

Gene Information

Description Not Available

Gene Symbol:   TSPY2
Gene Name:   testis specific protein, Y-linked 2
Aliases:   MGC103998, TSPYQ1
RefSeq:   NC_000024.9 NT_011896.9
Ensembl:   ENSG00000168757
Entrez:   64591
UniGene:   Hs.646249
Chromosome Mapping:   Yp11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.970000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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