PrimePCR™ SYBR® Green Assay: VARS2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039820

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VARS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VARS2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037097
Assay Design:   exonic
Chromosome Location:   6:30884882-30884986question
Amplicon Length:   75
Splice Variants Targeted:   ENST00000216194 ENST00000263335 ENST00000397647 ENST00000348715 ENST00000429538 ENST00000415079 ENST00000411697 ENST00000546854 ENST00000551709 ENST00000450090 ENST00000446577 ENST00000549458 ENST00000547340 ENST00000428577 ENST00000425563 ENST00000548165 ENST00000548842 ENST00000321897 ENST00000428017 ENST00000541562 ENST00000416670 ENST00000542001 ENST00000383369 ENST00000431578 ENST00000553245 ENST00000549900 ENST00000383540 ENST00000452966 ENST00000552560 ENST00000550773 ENST00000550037

Gene Information

Description Not Available

Gene Symbol:   VARS2
Gene Name:   valyl-tRNA synthetase 2, mitochondrial (putative)
Aliases:   MGC138259, MGC142165, VARS2L, VARSL
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000137411
Entrez:   57176
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.640000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download