PrimePCR™ SYBR® Green Assay: NRM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039819

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NRM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NRM, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037096
Assay Design:   exonic
Chromosome Location:   6:30656440-30656578question
Amplicon Length:   109
Splice Variants Targeted:   ENST00000444096 ENST00000376420 ENST00000259953 ENST00000376421 ENST00000431322 ENST00000449041 ENST00000434776 ENST00000427944 ENST00000422664 ENST00000446443 ENST00000422181 ENST00000450138 ENST00000411883 ENST00000422747 ENST00000445058 ENST00000444252 ENST00000438502 ENST00000456720 ENST00000436892 ENST00000383571 ENST00000383570 ENST00000430286

Gene Information

Description Not Available

Gene Symbol:   NRM
Gene Name:   nurim (nuclear envelope membrane protein)
Aliases:   NRM29
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1
Ensembl:   ENSG00000137404
Entrez:   11270
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.730000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download