PrimePCR™ SYBR® Green Assay: ATAT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039818

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATAT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATAT1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037095
Assay Design:   exonic
Chromosome Location:   6:30610628-30610774question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000449905 ENST00000442844 ENST00000430611 ENST00000423654 ENST00000416435 ENST00000428764 ENST00000547124 ENST00000416917 ENST00000432356 ENST00000454987 ENST00000411724 ENST00000457334 ENST00000456215 ENST00000550031 ENST00000443114 ENST00000454794 ENST00000443391 ENST00000446100 ENST00000448670 ENST00000456704 ENST00000553018 ENST00000423338 ENST00000445486 ENST00000424121 ENST00000457161 ENST00000425448 ENST00000450003 ENST00000553093 ENST00000397708 ENST00000291688 ENST00000383584 ENST

Gene Information

Description Not Available

Gene Symbol:   ATAT1
Gene Name:   alpha tubulin acetyltransferase 1
Aliases:   C6orf134, DKFZp547J097, FLJ13158, MEC17, Nbla00487, TAT
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000137343
Entrez:   79969
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download