PrimePCR™ SYBR® Green Assay: PPAN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039808

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PPAN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PPAN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037085
Assay Design:   exonic
Chromosome Location:   19:10218664-10218775question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000393976 ENST00000254043 ENST00000332582 ENST00000310325 ENST00000378466 ENST00000435556 ENST00000379264 ENST00000379255 ENST00000296869 ENST00000379246 ENST00000379244 ENST00000379240 ENST00000431707 ENST00000379249 ENST00000379272 ENST00000357096 ENST00000543479 ENST00000544770 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST00000469640 ENST00000268482 ENST00000280706 ENST00000367204 ENST00000372872 ENST00000394810 ENST00000537432 ENST00000350210 ENST00000368368 ENST

Gene Information

The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11) an adjacent downstream gene that encodes an ATP receptor has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation. [provided by RefSeq Nov 2010]

Gene Symbol:   PPAN
Gene Name:   peter pan homolog (Drosophila)
Aliases:   BXDC3, MGC14226, MGC45852, SSF, SSF-1, SSF1, SSF2
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000130810
Entrez:   56342
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 42.030000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download