PrimePCR™ SYBR® Green Assay: KIAA1267, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039796

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIAA1267, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIAA1267, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037073
Assay Design:   exonic
Chromosome Location:   17:44249182-44249362question
Amplicon Length:   151
Splice Variants Targeted:   ENST00000552923 ENST00000437635 ENST00000380099 ENST00000318217 ENST00000394649 ENST00000579584 ENST00000452329 ENST00000551851 ENST00000547336 ENST00000270458 ENST00000370801 ENST00000430726 ENST00000550381 ENST00000552452 ENST00000526025 ENST00000438725 ENST00000547402 ENST00000550140 ENST00000438415 ENST00000546928 ENST00000551633 ENST00000375688 ENST00000375686 ENST00000447450 ENST00000355303 ENST00000530676 ENST00000531349 ENST00000538023 ENST00000398543 ENST00000452630 ENST00000550450 ENST

Gene Information

Description Not Available

Gene Symbol:   KIAA1267
Gene Name:   KIAA1267
Aliases:   KANSL1, DKFZp686P06109, DKFZp727C091, MGC102843, MSL1v1
RefSeq:   NG_007398.1 NC_000017.10 NT_010783.15 NT_167251.1
Ensembl:   ENSG00000120071
Entrez:   284058
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.150000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download