PrimePCR™ SYBR® Green Assay: VTN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039788

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VTN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VTN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037065
Assay Design:   exonic
Chromosome Location:   17:26691499-26691639question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000572444 ENST00000571396 ENST00000536498 ENST00000438614 ENST00000431468 ENST00000555059 ENST00000333479 ENST00000252575 ENST00000230859 ENST00000552438

Gene Information

Homeodomain proteins such as SEBOX play a key role in coordinating gene expression during development (Cinquanta et al. 2000 [PubMed 10922053]).[supplied by OMIM Mar 2008]

Gene Symbol:   VTN
Gene Name:   vitronectin
Aliases:   V75, VN, VNT
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000109072
Entrez:   7448
Chromosome Mapping:   17q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.100000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download