PrimePCR™ SYBR® Green Assay: AKIRIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039551

List Price:    $142.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036827
Assay Design:   exonic
Chromosome Location:   1:39469834-39469978question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000432648 ENST00000446189 ENST00000372984 ENST00000531822

Gene Information

Description Not Available

Gene Symbol:   AKIRIN1
Gene Name:   akirin 1
Aliases:   C1orf108, FLJ12666, RP11-781D11.2, STRF2
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000174574
Entrez:   79647
UniGene:   Hs.293563
Chromosome Mapping:   1p34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.050000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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