This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0039415
PrimePCR™ PreAmp for SYBR® Green Assay: RBP7, Human
PrimePCR™ Template for SYBR® Green Assay: RBP7, Human
Due to its chemical instability and low solubility in aqueous solution vitamin A requires cellular retinol-binding proteins (CRBPs) such as RBP7 for stability internalization intercellular transfer homeostasis and metabolism.[supplied by OMIM May 2004]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.