PrimePCR™ SYBR® Green Assay: SLC1A3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039065

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SLC1A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SLC1A3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036341
Assay Design:   exonic
Chromosome Location:   5:36608431-36608525question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000265113 ENST00000513903 ENST00000416645 ENST00000505202 ENST00000513646 ENST00000381918

Gene Information

This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq Nov 2009]

Gene Symbol:   SLC1A3
Gene Name:   solute carrier family 1 (glial high affinity glutamate transporter), member 3
Aliases:   EA6, EAAT1, FLJ25094, GLAST, GLAST1
RefSeq:   NC_000005.9 NT_006576.16 NG_015890.1
Ensembl:   ENSG00000079215
Entrez:   6507
Chromosome Mapping:   5p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.710000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download