PrimePCR™ SYBR® Green Assay: CYTH3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0039018

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CYTH3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYTH3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0036294
Assay Design:   exonic
Chromosome Location:   7:6204928-6205055question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000350796 ENST00000396741

Gene Information

This gene encodes a member of the PSCD (pleckstrin homology Sec7 and coiled-coil domains) family. PSCD family members have identical structural organization that consists of an N-terminal coiled-coil motif a central Sec7 domain and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This encoded protein is involved in the control of Golgi structure and function and it may have a physiological role in regulating ADP-ribosylation factor protein 6 (ARF) functions in addition to acting on ARF1. [provided by RefSeq Jul 2008]

Gene Symbol:   CYTH3
Gene Name:   cytohesin 3
Aliases:   ARNO3, GRP1, PSCD3
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000008256
Entrez:   9265
Chromosome Mapping:   7p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download