PrimePCR™ SYBR® Green Assay: MTR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0036114

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MTR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MTR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0035289
Assay Design:   Exonic
Chromosome Location:   1:237054439-237054569question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000366577 ENST00000366576 ENST00000535889

Gene Information

MTR encodes the enzyme 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme also known as cobalamin-dependent methionine synthase catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. [provided by RefSeq Jul 2008]

Gene Symbol:   MTR
Gene Name:   5-methyltetrahydrofolate-homocysteine methyltransferase
Aliases:   FLJ33168, FLJ43216, FLJ45386, MS, cblG
RefSeq:   NC_000001.10 NG_008959.1 NT_167186.1
Ensembl:   ENSG00000116984
Entrez:   4548
Chromosome Mapping:   1q43

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download