This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0035550
PrimePCR™ PreAmp for SYBR® Green Assay: SOSTDC1, Human
PrimePCR™ Template for SYBR® Green Assay: SOSTDC1, Human
This gene is a member of the sclerostin family and encodes an N-glycosylated secreted protein with a C-terminal cystine knot-like domain. This protein functions as a bone morphogenetic protein (BMP) antagonist. Specifically it directly associates with BMPs prohibiting them from binding their receptors thereby regulating BMP signaling during cellular proliferation differentiation and programmed cell death. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.