PrimePCR™ SYBR® Green Assay: ZNF43, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ZNF43, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ZNF43, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0034376
Assay Design:   Exonic
Chromosome Location:   19:22033740-22033913question
Amplicon Length:   144

Gene Information

This gene belongs to the C2H2-type zinc finger gene family. The zinc finger proteins are involved in gene regulation and development and are quite conserved throughout evolution. Like this gene product a third of the zinc finger proteins containing C2H2 fingers also contain the KRAB domain which has been found to be involved in protein-protein interactions. [provided by RefSeq Jul 2008]

Gene Symbol:   ZNF43
Gene Name:   zinc finger protein 43
Aliases:   DKFZp686L1854, HTF6, KOX27, ZNF39L1
RefSeq:   NC_000019.9 NT_011295.11 NW_003315964.1
Ensembl:   ENSG00000198521
Entrez:   7594
Chromosome Mapping:   19p13.1-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.650000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download