PrimePCR™ SYBR® Green Assay: PARP1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: PARP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PARP1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0023747
Assay Design:   Exonic
Chromosome Location:   1:226555181-226555308question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000366794

Gene Information

This gene encodes a chromatin-associated enzyme poly(ADP-ribosyl)transferase which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation proliferation and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition this enzyme may be the site of mutation in Fanconi anemia and may participate in the pathophysiology of type I diabetes. [provided by RefSeq Jul 2008]

Gene Symbol:   PARP1
Gene Name:   poly (ADP-ribose) polymerase 1
Aliases:   ADPRT, ADPRT 1, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143799
Entrez:   142
Chromosome Mapping:   1q41-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.410000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download