PrimePCR™ SYBR® Green Assay: AC104304.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: AC104304.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC104304.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0023686
Assay Design:   Exonic
Chromosome Location:   3:46623465-46623624question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000296145 ENST00000542931

Gene Information

This gene encodes an epidermal growth factor-related protein that contains a cripto FRL-1 and cryptic domain. The encoded protein is an extracellular membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2 3 6 8 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq Mar 2010]

Gene Symbol:   AC104304.1
Gene Name:   Putative teratocarcinoma-derived growth factor 2
Aliases:   CR, CRGF, CRIPTO
RefSeq:   NC_000003.11 NG_017049.1 NG_021214.1 NT_022517.18
Ensembl:   ENSG00000241186
Entrez:   6997
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.780000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download