PrimePCR™ SYBR® Green Assay: TAF1D, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TAF1D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TAF1D, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0023489
Assay Design:   Exonic
Chromosome Location:   11:93471336-93471465question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000448108 ENST00000532455

Gene Information

TAF1D is a member of the SL1 complex which includes TBP (MIM 600075) and TAF1A (MIM 604903) TAF1B (MIM 604904) and TAF1C (MIM 604905) and plays a role in RNA polymerase I transcription (Wang et al. 2004 [PubMed 15520167]; Gorski et al. 2007 [PubMed 17318177]).[supplied by OMIM Jun 2009]

Gene Symbol:   TAF1D
Gene Name:   TATA box binding protein (TBP)-associated factor, RNA polymerase I, D, 41kDa
Aliases:   JOSD3, MGC5306, RAFI41, TAF(I)41
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000166012
Entrez:   79101
Chromosome Mapping:   11q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.320000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download