This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0033804
PrimePCR™ PreAmp for SYBR® Green Assay: NFIL3, Human
PrimePCR™ Template for SYBR® Green Assay: NFIL3, Human
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells natural killer (NK) cells and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors such as NFIL3 that bind to regulatory regions of the gene usually upstream of the transcription start site (Zhang et al. 1995 [PubMed 7565758]).[supplied by OMIM Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.