PrimePCR™ SYBR® Green Assay: CITED4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0032461

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CITED4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CITED4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0019600
Assay Design:   Exonic
Chromosome Location:   1:41326949-41327040question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000372638

Gene Information

The protein encoded by this intronless gene belongs to the CITED family of transcriptional coactivators that bind to several proteins including CREB-binding protein (CBP) and p300 via a conserved 32 aa C-terminal motif and regulate gene transcription. This protein also interacts with transcription factor AP2 (TFAP2) and thus may function as a co-activator for TFAP2. Hypermethylation and transcriptional downregulation of this gene has been observed in oligodendroglial tumors with deletions of chromosomal arms 1p and 19q and associated with longer recurrence-free and overall survival of patients with oligodendroglial tumors. [provided by RefSeq Aug 2011]

Gene Symbol:   CITED4
Gene Name:   Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 4
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000179862
Entrez:   163732
Chromosome Mapping:   1p34.2,1p35-p34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download