This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCEP0032449
PrimePCR™ PreAmp for SYBR® Green Assay: TCEB3B, Human
PrimePCR™ Template for SYBR® Green Assay: TCEB3B, Human
This gene encodes the transcriptionally active subunit of the SIII (or elongin) transcription elongation factor complex which also includes two regulatory subunits elongins B and C. This complex acts to increase the rate of RNA chain elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites along the DNA template. Whereas a related protein with similar function elongin A is ubiquitously expressed the encoded protein is specifically expressed in the testis suggesting it may have a role in spermatogenesis. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.