PrimePCR™ SYBR® Green Assay: SUMO4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0032415

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SUMO4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SUMO4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0019518
Assay Design:   Exonic
Chromosome Location:   6:149721704-149721806question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000326669

Gene Information

This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization stability or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene which leads to the M55V substitution has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq Jul 2008]

Gene Symbol:   SUMO4
Gene Name:   SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
Aliases:   IDDM5, SMT3H4, SUMO-4, dJ281H8.4
RefSeq:   NC_000006.11 NG_012301.1 NG_021386.1 NT_025741.15
Ensembl:   ENSG00000177688
Entrez:   387082
Chromosome Mapping:   6q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.390000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download