PrimePCR™ SYBR® Green Assay: LRRC23, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007049
Assay Design:   Exonic
Chromosome Location:   12:7014788-7014894question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000433346 ENST00000007969 ENST00000323702 ENST00000443597 ENST00000415834 ENST00000436789 ENST00000429740

Gene Information

Description Not Available

Gene Symbol:   LRRC23
Gene Name:   leucine rich repeat containing 23
Aliases:   LRPB7
RefSeq:   NC_000012.11 NT_009759.16
Ensembl:   ENSG00000010626
Entrez:   10233
UniGene:   Hs.155586
Chromosome Mapping:   12p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996500
y-intercept 34.030000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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