PrimePCR™ SYBR® Green Assay: P2RY13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0007014
Assay Design:   Exonic
Chromosome Location:   3:151044208-151044332question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000325602

Gene Information

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity which overlaps in some cases for various adenosine and uridine nucleotides. This receptor is activated by ADP. [provided by RefSeq Sep 2008]

Gene Symbol:   P2RY13
Gene Name:   purinergic receptor P2Y, G-protein coupled, 13
Aliases:   GPCR1, GPR86, GPR94, P2Y13, SP174
RefSeq:   NC_000003.11 NG_021244.1 NT_005612.16
Ensembl:   ENSG00000181631
Entrez:   53829
UniGene:   Hs.546396
Chromosome Mapping:   3q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.300000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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