PrimePCR™ SYBR® Green Assay: CYorf15A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025457

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005678
Assay Design:   Exonic
Chromosome Location:   Y:21750317-21750485question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000538014 ENST00000447202

Gene Information

Description Not Available

Gene Symbol:   CYorf15A
Gene Name:   chromosome Y open reading frame 15A
Aliases:   FLJ33216, MGC131732
RefSeq:   NC_000024.9 NT_011875.12
Ensembl:   ENSG00000099749
Entrez:   246126
UniGene:   Not Available
Chromosome Mapping:   Yq11.222

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.310000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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