PrimePCR™ SYBR® Green Assay: LRCH4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005676
Assay Design:   Exonic
Chromosome Location:   7:100183509-100183601question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000310300

Gene Information

This gene encodes a protein that contains leucine-rich repeats (LRR) at its amino terminus and that is known to be involved in ligand binding. The carboxyl terminus may act as a membrane anchor. Identified structural elements suggest that the encoded protein resembles a receptor. [provided by RefSeq Jul 2008]

Gene Symbol:   LRCH4
Gene Name:   leucine-rich repeats and calponin homology (CH) domain containing 4
Aliases:   FLJ40101, FLJ46315, LRN, LRRN1, LRRN4, PP14183
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000077454
Entrez:   4034
UniGene:   Hs.125742
Chromosome Mapping:   7q22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.870000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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