PrimePCR™ SYBR® Green Assay: MTHFR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025904

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MTHFR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MTHFR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005433
Assay Design:   Exonic
Chromosome Location:   1:11850746-11850867question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000376592 ENST00000376590 ENST00000376585 ENST00000376583

Gene Information

The protein encoded by this gene catalyzes the conversion of 510-methylenetetrahydrofolate to 5-methyltetrahydrofolate a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular disease neural tube defects colon cancer and acute leukemia and mutations in this gene are associated with methylenetetrahydrofolate reductase deficiency.[provided by RefSeq Oct 2009]

Gene Symbol:   MTHFR
Gene Name:   methylenetetrahydrofolate reductase (NAD(P)H)
Aliases:   Not Available
RefSeq:   NC_000001.10 NG_008766.1 NG_013351.1 NT_021937.19
Ensembl:   ENSG00000177000
Entrez:   4524
Chromosome Mapping:   1p36.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.500000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download