PrimePCR™ SYBR® Green Assay: MAST4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025324

List Price:    $146.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005230
Assay Design:   Exonic
Chromosome Location:   5:66459240-66459381question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000403625 ENST00000403666 ENST00000405643 ENST00000261569 ENST00000443808 ENST00000404260 ENST00000380908 ENST00000432399

Gene Information

Description Not Available

Gene Symbol:   MAST4
Gene Name:   microtubule associated serine/threonine kinase family member 4
Aliases:   DKFZp686E18148, DKFZp686N1467, FLJ16540, FLJ33039, KIAA0303
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000069020
Entrez:   375449
UniGene:   Hs.595458
Chromosome Mapping:   5q12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.750000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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