PrimePCR™ SYBR® Green Assay: C14orf93, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025299

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005163
Assay Design:   Exonic
Chromosome Location:   14:23456755-23457121question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000299088 ENST00000341470 ENST00000397379 ENST00000397382 ENST00000397377 ENST00000406429

Gene Information

Description Not Available

Gene Symbol:   C14orf93
Gene Name:   chromosome 14 open reading frame 93
Aliases:   FLJ12154
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000100802
Entrez:   60686
UniGene:   Hs.255874
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.240000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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