PrimePCR™ SYBR® Green Assay: AHRR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: AHRR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AHRR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005113
Assay Design:   Exonic
Chromosome Location:   5:434352-434461question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000505113 ENST00000316418 ENST00000512529 ENST00000506456 ENST00000511487

Gene Information

The protein encoded by this gene participates in the aryl hydrocarbon receptor (AhR) signaling cascade which mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. It functions as a feedback modulator by repressing AhR-dependent gene expression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Jun 2011]

Gene Symbol:   AHRR
Gene Name:   aryl-hydrocarbon receptor repressor
Aliases:   AHH, AHHR, KIAA1234, MGC167813, MGC176630, bHLHe77
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000063438
Entrez:   57491
Chromosome Mapping:   5p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.870000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download