PrimePCR™ SYBR® Green Assay: STEAP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004938
Assay Design:   Exonic
Chromosome Location:   2:120022323-120022496question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000393110 ENST00000393106 ENST00000393108 ENST00000354888 ENST00000425223 ENST00000546236

Gene Information

Description Not Available

Gene Symbol:   STEAP3
Gene Name:   STEAP family member 3
Aliases:   STMP3, TSAP6, dudlin-2
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000115107
Entrez:   55240
UniGene:   Hs.647822
Chromosome Mapping:   2q14.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.590000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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