PrimePCR™ SYBR® Green Assay: SNF8, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025192

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SNF8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SNF8, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004893
Assay Design:   Exonic
Chromosome Location:   17:47007848-47007973question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000502492 ENST00000290330

Gene Information

SNF8 VPS25 (MIM 610907) and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II) a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8 VPS25 and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al. 2005 [PubMed 15755741]; Kamura et al. 2001 [PubMed 11278625]).[supplied by OMIM Mar 2008]

Gene Symbol:   SNF8
Gene Name:   SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
Aliases:   Dot3, EAP30, VPS22
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000159210
Entrez:   11267
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.790000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download