PrimePCR™ SYBR® Green Assay: RBMXL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0025146

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RBMXL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RBMXL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004785
Assay Design:   Exonic
Chromosome Location:   1:89447293-89447454question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000321792 ENST00000399794

Gene Information

This gene represents a retrogene of RNA binding motif protein X-linked (RBMX) which is located on chromosome X. While all introns in the coding sequence have been processed out compared to the RBMX locus the ORF is intact and there is specific evidence for transcription at this location. The preservation of the ORF by purifying selection in all Old World monkeys carrying it suggests that this locus is likely to be functional possibly during male meiosis when X chromosomal genes are silenced or during haploid stages of spermatogenesis. This gene shares 5' exon structure with the cysteine conjugate-beta lyase 2 locus on chromosome 1 but the coding sequences are non-overlapping. Alternative splicing results in two transcript variants. [provided by RefSeq Jun 2009]

Gene Symbol:   RBMXL1
Gene Name:   RNA binding motif protein, X-linked-like 1
Aliases:   DKFZp547N1117, DKFZp667D0223, FLJ32614, RBM1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000213516
Entrez:   494115
Chromosome Mapping:   1p22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.100000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download