PrimePCR™ SYBR® Green Assay: MSX1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0004685
Assay Design:   Exonic
Chromosome Location:   4:4865106-4865203question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000382723

Gene Information

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation craniofacial development particularly odontogenesis and tumor growth inhibition. Mutations in this gene which was once known as homeobox 7 have been associated with nonsyndromic cleft lip with or without cleft palate 5 Witkop syndrome Wolf-Hirschom syndrome and autosomoal dominant hypodontia. [provided by RefSeq Jul 2008]

Gene Symbol:   MSX1
Gene Name:   msh homeobox 1
Aliases:   HOX7, HYD1, STHAG1
RefSeq:   NC_000004.11 NG_008121.1 NT_006051.18
Ensembl:   ENSG00000163132
Entrez:   4487
UniGene:   Hs.424414
Chromosome Mapping:   4p16.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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